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Cholesteryl hemisuccinate tris salt anionic detergent ...- chs detergente anatrace ,Cholesteryl hemisuccinate is an ionizable anionic detergent that stabilizes large unilamellar vesicles. These liposome vesicles act as delivery agents for anticancer drugs, oligonucleotides and antibiotics. Cholesteryl succinate also reportedly exhibits antiproliferative activity and may be …Sample preparation for structural and functional analyses ...Jan 01, 2020·3% Lauryl maltose neopentyl glycol/0.3% cholesteryl hemisuccinate tris salt detergent solution in water (LMNG and CHS both from: Anatrace). 11. LMNG detergent buffer: 20 mM Hepes pH 7.5, 200 mM NaCl, 1% LMNG/0.1% CHS. 12. Wash buffer: 20 mM Hepes pH 7.5, 200 mM NaCl, 60 mM imidazole, 0.1% LMNG/0.01% CHS. 13.



Structure of the human voltage-gated sodium channel Nav1.4 ...

The suspension was supplemented with 1% (w/v) n-dodecyl-β-d-maltopyranoside (DDM, Anatrace), 0.2% (w/v) cholesteryl hemisuccinate Tris salt (CHS, Anatrace), 0.001% (w/v) dodecyl sulfate sodium salt (SDS, BIO-RAD), and protease inhibitor cocktail including 2 mM phenylmethylsulfonyl fluoride (PMSF), 6.5 μg/ml aprotinin, 3.5 μg/ml pepstatin ...

Anatrace Maltosides

D310-CH210 10 ML. 10:1 DDM/CHS Pre-Made Solution (10 x 1 ml) Units Add to cart. D322LA 1 GM. n-Decyl-β-D-Maltopyranoside, Anagrade

Anatrace Neopentyl Glycol Class Detergents

Typical working concentrations: 1% LMNG, 0.1% CHS for protein extraction and 0.01% LMNG, 0.001% CHS for protein purification and structure determination. Appearance – Clear liquid Quality Assurance Tested – tested to 5 freeze thaw cycles Read more about our LMNG:CHS Premixed Solution in our May, 2018 Newsletter.

Anatrace Detergents

Anatrace has been the leader in high-purity detergents and high standards since the late 1980s. Since the launch of our first detergent, Dodecyl Maltoside (DDM), our portfolio has grown into the largest in the industry – with more than 50 unique detergents.

Probing thermostability of detergent-solubilized CB2 ...

Jan 03, 2020·Streptactin XT was from IBA Life Sciences (Goettingen, Germany). The detergents CHAPS, LMNG, and DDM were from Anatrace (Maumwee, OH). CHS–Tris salt was from Anatrace. The detergent Façade–TEG (3a,7a,12a-tri-((O-β-d-glucopyranosyl)ethyloxy)-cholane) was from Avanti Polar Lipids (Alabaster, AL). The potent nonselective CB 2 cis-3[2-hydroxy ...

Dissolving Cholesteryl hemisuccinate tris salt in detergents?

The description from Anatrace doesn't really say much except that it's soluble to 1.2% in 6% CHAPS. Sigma also say that it is soluble in methanol to 1%. ... the ratio is 10% detergent to 2 % CHS ...

Acquires Microlytic — CalibreScientific

About Anatrace. Anatrace is a developer, manufacturer, and distributor of approximately 250 high-purity detergents and synthetic lipids for use in membrane protein research studies headquartered in …

Anatrace Cholesterol Derivatives

1919. Product Description Technical Documentation Required / Related Products. Molecular Formula: C 31 H 50 O 4 · C 4 H 11 NO 3. CAS Number: [102601-49-0] Formula Weight: 607.9. Identity: IR spectrum confirms to specification. Solubility: 1.2% in a 6% water solution of CHAPS. Appearance: White powder. DSC confirms to standard.

Ddm | anatrace | Bioz

Figure Legend Snippet: Fig. 5. The effect of MTSET on transport activity in proteoliposomes. Wild-type OpuA and OpuA(W484C) were incubated with freshly prepared 100 µM MTSET for 1 h at room temperature while bound to the Ni-NTA resin, which was followed by an additional 20 column volumes of washing with 50 mM KPi pH 8.0, 200 mM KCl, 20% glycerol, 0.05% DDM and 15 mM imidazole; the ...

Incorporation of cholesteryl hemisuccinate into detergent ...

incorporation of cholesterol or cholesterol analogs into detergent micelles at a high enough concentration to be useful in purification protocols is often not straightforward due to the ability of different detergents to solubilize cholesterol. Materials: 1. Reagents: n-dodecyl-B-d-maltopyranoside (Anatrace D310)

Cholesteryl Hemisuccinate | Anatrace | Bioz

Figure Legend Snippet: Structure of GIRK2 with PIP 2 and cholesteryl hemisuccinate (CHS) (A) GIRK2 PIP2/CHS cryoEM map; one CHS (green, at “site A”) molecule per subunit is modeled into planar densities on the opposite side of the PIP 2 (red)-channel interface; another planar density at “site B” near the slide helix is shown in orange. GIRK2 subunits are in blue with one highlighted in ...

Assemblies of lauryl maltose neopentyl glycol (LMNG) and ...

May 01, 2019·1. Introduction. Detergents are amphipatic molecules that auto-assemble into micelles above the critical micelle concentration (CMC). Above the CMC, they are able to solubilize lipids and membrane proteins, forming protein-detergent complexes of small and well-defined size [].Detergents are essential for membrane protein solubilization and purification steps, as well as functional and ...

GPCR stabilization using the bicelle-like architecture of ...

Dec 01, 2011·ORL-1 was expressed in Sf9 insect cells, and a thorough search for optimal detergents identified DDM as the best detergent for extraction and purification of this receptor (data not shown). To date, little is known about the thermostabilizing effect of sterol addition to OR PDCs. Because cholesterol is insoluble in the absence of lipids (unpublished observations), we tested the effect of ...

High-throughput stability screening for detergent ...

Jul 17, 2019·Protein stability in detergent or membrane-like environments is the bottleneck for structural studies on integral membrane proteins (IMP). ... (w/v) DDM, 0,005% (w/v) CHS Anatrace …

Anatrace November/December Newsletter. Higher standards ...

Anatrace is fully committed to synthesizing these reagents, and our newer detergents, including LMNG and Amphipol A8-35, have been highly successful in the hands of researchers. Another recent addition to the Anatrace product line, glyco-diosgenin (GDN) (1) is also showing great promise, and can be tried in parallel or after the standard ...

Ch210-5gm | cholesteryl hemisuccinate tris salt Biotrend

CHS. CH210: Cholesteryl Hemisuccinate Tris Salt. Molecular Formula: C 31 H 50 O 4 · C 4 H 11 NO 3. CAS Number: [102601-49-0] Formula Weight: 607.9. Identity: IR spectrum confirms to specification. Solubility: 1.2% in a 6% water solution of CHAPS.

Anatrace. Higher standards for detergents, lipids and ...

At Anatrace, we’re seriously committed to developing and supplying the industry’s finest high-purity detergents and lipids – and equally committed to the high standards that make it possible. Our standards have made Anatrace an internationally-recognized leader in manufacturing reagents for membrane protein studies and custom chemical ...

Sample preparation for structural and functional analyses ...

Jan 01, 2020·3% Lauryl maltose neopentyl glycol/0.3% cholesteryl hemisuccinate tris salt detergent solution in water (LMNG and CHS both from: Anatrace). 11. LMNG detergent buffer: 20 mM Hepes pH 7.5, 200 mM NaCl, 1% LMNG/0.1% CHS. 12. Wash buffer: 20 mM Hepes pH 7.5, 200 mM NaCl, 60 mM imidazole, 0.1% LMNG/0.01% CHS. 13.

Anatrace社 Anatrace Products | A must-read for membrane ...

It is easy to see that the vast majority of GPCR structures were solved using X-ray crystallography with maltoside+CHS as both the predominant solubilization agent and the main detergent used in structure determination, with MNG+CHS mix taking second place. This paper is an invaluable guide for anyone setting out to solve a membrane protein ...

How do you choose the detergent concentration for protein ...

Jan 01, 2009·Hi, I am trying to prepare a series of buffers for protein purification work, and planning to add detergent (2% DDM, and 0.5% CHS) to extract membrane proteins from cell lysates.

200x DDM CHS buffer - ACROBiosystems

This 200x detergent solution contains 10% (w/v) DDM and 2% (w/v) CHS that is necessary for solubilization of membrane proteins. DDM, or n-Dodecyl β-D-Maltoside [CAS: 69227-93-6], is a maltoside based non-ionic detergent with a hydrophilic maltose head and a hydrophobic long chain alkyl tail. CHS, or Cholesteryl Hemisuccinate Tris Salt [CAS ...

Molecular basis for kinin selectivity and activation of ...

Sep 09, 2021·The detergent of washing buffer was then displaced by 0.1% (w/v) lauryl maltose neopentylglycol (LMNG, Anatrace) and 0.02% (w/v) CHS for 10 column volumes washing, followed by 0.03% (w/v) LMNG, 0 ...

Structural basis of human ghrelin receptor signaling by ...

Nov 04, 2021·The detergent was exchanged to 0.01% (w/v) lauryl maltose neopentyl glycol (MNG, Anatrace) on the M1 antibody resin. To do so, the column …

Anatrace Anapoe Detergents

Anapoe-20. APT080 - Anapoe-80 APT080. Anapoe-80. APX100 - Anapoe-X-100 APX100. Anapoe-X-100. APX114 - Anapoe-X-114 APX114. Anapoe-X-114. APX305 - Anapoe-X …

Anatrace C322说明书-上海起发实验试剂有限公司

美国Anatrace成立于1984年.公认的膜蛋白研究用产品供应商,为膜蛋白提取与纯化提供高纯度生化产品,包括了各种表面活性剂、试剂盒及各种研究用工具书。提供膜蛋白提取和纯化试剂,高纯的生化试剂和清洁剂,去垢剂等 产品分类: 1.Nonionic detergents-非离子去垢 ...

DETERGENTS AND THEIR USES IN MEMBRANE PROTEIN …

as detergents—a polar hydrophilic head group and a nonpolar hydrophobic tail—lipids differ from detergents in the shape of the monomers, in the type Hydrophilic head group Hydrophobic tail WWW.ANATRACEOM I. INTRODUCTION Membrane protein studies have advanced significantly over the past few years. This is partly due to advances in tools ...